Effect of Water Stress on Biochemical Metabolites in Fenugreek (Trigonella Foenum-Graecum L.) Genotypes
Keywords:
Fenugreek, water stress, antioxidants, proline, glycine betaine, Glutathione Reduced, peroxidase activity
Abstract
A field experiment was conducted at Agronomy farm, S.K.N. Collage of Agriculture, Jobner during Rabi season 2014-2015 to investigate “Biochemical changes in fenugreek (Trigonella foenum graecum L.) genotypes under water stress” using Five genotypes of fenugreek (UM-100,UM-112,UM-124,UM-134,UM-140). The significant increment observed in proline content, Glycine betaine content and Peroxidase activity due to water stress at both the stages. A Reduction in the content of total chlorophyll and GSH in stressed plants of all genotypes was recorded at both stages. Significant reductions in total carotenoids content were found in all the genotypes at 65 DAS under water stress conditions. The MDA values in stressed plants were found higher over respective controls at both stages in all the genotypes.
References
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2. Aggrwal, K.B., Ranjan, J.K., Rathore, S.S., Saxena, S.N. and Mishra, B.K. 2013. Changes in physical and biochemical properties of fenugreek (Trigonella species L.) leaf during different growthstages. International Journal of Seed Spices, 3(1): 31-35.
3. Asada, K. and Takahashi, M. 1987. Production and scavenging of active oxygen in photosynthesis. Elsevier, pp. 227-287.
4. Bailey, C. 1998. Free radical scavengers as affected by accelerated ageing in subsequent priming in sunflower seeds. Physiologia Plantarum, 104: 646-52.
5. Bates, L.S., Waldren, R.P. and Teare, I.D. 1973. Rapid determination of free proline for water stress studies. Plant and Soil, 39: 205-207.
6. Benjamin, J.G. and Nielsen, D.C. 2006. Water deficit effects on root distribution of soyabean, fieldpea and chickpea. Field Crop Research, 97: 248-253.
7. Boyer, J.S. 1982. Plant Productivity and Environment Science, 218: 443-448.
8. Costa, H., Gallego, S.M. and Tomaro, M.L. 2002. Effect of UV-B radiation on antioxidant defence system in sunflower cotyledons. Plant Sciences, 62: 939-945.
9. Ekmekc, I.Y., Bohms, A., Thomson, J.A. and Mundree, S.G. 2005. Photochemical and Xerophyta viscosa Baker and Digitaria sanguinalis L. under water deficit. Naturforsch. 60c: 435-443.
10. Grieve, C.M. and Grattan, S.R. 1983. Rapid assay for determination of Water soluble quaternary ammonium compounds. Plant and Soil, 70: 303-307.
11. Heath, R.I. and Packer, L. 1968. Photoperoxidation in isolated chloroplast. Kinetics and stoichiometry of fatty acid peroxidation. Archive Biochemistry Biophysics, 125:189-198.
12. Hiscox, J.D. and Israelstam. G.F. 1979. A method for the extraction ofchlorophyll from leaf tissue without maceration. Canadian Journal of Botany,57: 1332-34.
13. Hitz, W.D. and Hanson, A.D. 1980. Determination of glycine betaine by pyrolysis-gas chromatography in cereals and grasses. Phytochemistr, 19: 2371- 2374.
14. Karmakar, N., Chakravarty, A., Bandhopadhay, P.K. and Das, P.K. 2014. Response of fenugreek (Trigonella foenum-graecum L.) seedling under moisture and heavy metal stress with special reference to antioxidant system. African Journal of Bio- Technology, 13(3): 434-440.
15. Mittal, G.K., Joshi, A., Rajamani, G., Mathur, P.N. and Sharma, A. 2006. Water deficit induced germination of reactive oxygen species and antioxidants in two Spanich groundnut cultivars. National Journal of plant Improvement, 8: 7-10.
16. Mittal, G.K. 2010. Biochemical and molecular studies in Maize (Zea mays L.) genotypes for water stress tolerance. Ph. D. Thesis submitted to Anand Agriculture University, Anand ( Gujrat ).
17. Pant, C.N., Agarrwal, R. and Agrawal, S. 2014. Mannitol-induced drought stress on calli of Trigonella foenum-graecum L. var. RMt-303. Indian Journal of Experimental Biology, 52: 1128-1137.
18. Praba, M.L., Cairns, J.E., Babu, R.C. and Lafitte, H.R. 2009. Identification of physiological traits underlying cultivar differences in drought tolerance in rice and wheat. Crop Science,195: 30-46.
19. Ranganayakulu, G.S., Sudhakar, C. and Reddy, S. 2015. Effect of water stress on proline metabolism and leaf relative water content in two high yielding genotypes of groundnut (Arachis hypogea L.) with contrasting drought tolarence. Journal of Experimental Biology and Agricultural Sciences, 3 (1): 2320-8694.
20. Rao, P.U. and Sharma, R.D. 1987. An evaluation of protein quality in fenugreek seed and their supplementary effect. Food Chemistry, 24: 1-9.
21. Rhodes, D., Rich, P.J., Myers, A.C., Rueter, C.C. and Jamieson, G.C. 1987. Determination of Betaines by fast atom bombardment mass spectrometry: identification of glycine betaine deficient genotypes of Zea mays L. Plant Physiology, 84: 781-788.
22. Mishra, M., Das, N. and Mishra, A.N. 1995. NaCl salt stress induced changes in protein and protease activity of pearl millet callus.Acta Physiology of Plant, 17: 371.
23. Wyn Jones, R.G. and Storey, R. 1981. Betaines. In: Paleg LG, Aspinall D (Eds.), The Physiology and Biochemistry of Drought Resistance in Plants. Academic Press, Sydney, pp. 171–204.
24. Wellburn, A.R. 1994. The spectral determination of chlorophyll a and b, as well as carotenoides using various solvent with spectrophotometers of different resolution. Journal of Plant Physiology,144: 307-313.
Published
2023-03-09
How to Cite
Meena, S., Meena, A. K., Meena, S., & Meena, D. (2023). Effect of Water Stress on Biochemical Metabolites in Fenugreek (Trigonella Foenum-Graecum L.) Genotypes. International Journal on Orange Technologies, 5(3), 11-18. Retrieved from https://journals.researchparks.org/index.php/IJOT/article/view/4095
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Articles
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