Results of a study to determine the lethal doses (ld100 and ld50) of s. typhimurium in experiments on laying chickens

  • A.Kh.Khatamov Student of PhD,2 Doctor of Veterinary Sciences
  • H.S. Salimov Professor of Sam.I.V.M
Keywords: Poultry farming, S. enteritides, Toxicoinfection, LD50, Nutrient medium, Microbial bodies

Abstract

In laboratory experiments carried out on 12 day old chickens, the 100% lethal dose (LD100) was established in the amount of 600 million microbial bodies - S. typhimurium. Thus LD50 (killing 50% of chickens) amounted to 400 million Salmonella. The duration of the incubation period in the hyperacute course of experimental salmonellosis of chickens (5 heads) was 6 to 10 hours. Clinical signs and pathological changes characteristic of this disease were not observed. They died 8-12 hours after infection. The incubation period in acute course lasted from 10 hours to 14 hours. In two sick chickens, clinical signs of the disease began to appear after 16 hours, in four more - after 20-24 hours after infection. As a result of bacteriological examination of pathological material (parenchymal organs, tubular bone) of chickens with salmonellosis, cultures of S. typhimurium with all cultural, morphological, biochemical and tinctorial properties were re-isolated

References

Костенко, Ю.Г., Храмов, М.В., Давлеев, А.Д. Современные аспекты возникновения и предупреждения пищевого сальмонеллеза. // Ветеринария № 4, 2012. С. 9 – 13.
Кузьмин В.А. Сальмонеллёзная инфекция в условиях птицефабрики, методы профилактики и оздоровления: Автореф. дисс... докт. вет. наук. СПбАВМ. – СПб., 1995. – 16 с.
Сергевнин В. И. Научно-методические основы эпизоотолого-эпидемиологического надзора за сальмонеллёзной инфекцией //Эпидемиология и инфекционные болезни. – 1997. – №4. – С. 32-34.
Черкасский Б.Л. Современная эволюция сальмонеллезов // Тезисы докл. Междунар. симп. по пищевым зоонозам.– М.: МГМИ, 1995. – С. 18-19.
Butaeva I.М., Salimov H.S., Davlatov R.B. On The Diagnosis Of Mixed Bacterial Infections Of Birds. // International Journal of Advanced Science and Technology. Vol. 29, No. 9s, (2020), pp. 2308-2315.
Motarjemi Y., Kdferstein F.K., Moya G. et al. Importance of HACCP (Harard Analisis and Critical Control points) for Public Health and development. // The role of the world health organization: Food Control. –1997.– № 7.– P.77-85.
Published
2021-01-25
How to Cite
[1]
A.Kh.Khatamov and H.S. Salimov 2021. Results of a study to determine the lethal doses (ld100 and ld50) of s. typhimurium in experiments on laying chickens. International Journal on Integrated Education. 4, 1 (Jan. 2021), 164-168. DOI:https://doi.org/10.17605/ijie.v4i1.1154.
Section
Articles